Effect of an extender enriched with algerian date palm pollen on chilled semen characteristics of rabbit bucks at different ages
نویسندگان
چکیده
In the present study, we evaluated effect of various concentrations date palm pollen (DPP) aqueous extract on rabbit sperm during storage at 4°C for 48 h. Semen was collected from old and young bucks using an artificial vagina initially quality. The were diluted in Tris buffer supplemented with (20, 40, 80 mg/mL) DPP extract. extended samples stored Sperm motility motion kinetics assessed after 2, 4, 24 h storage. At each time, aliquot frozen analytical evaluation thiobarbituric acid reactive substances (TBARS) (lipid peroxidation) tocols (vitamin E). Proximate composition, antioxidant sugar content DPP. results showed that characterised by a high proportion protein (27.10%) ash (18.43%), whereas lipid fraction very low (0.51%) total also (16.25 g/100 g fresh matter, f.m.). Regarding antioxidants content, sum value 26.48 mg/g f.m. polyphenols 5.01 mg gallic equivalents/g f.m, polyunsaturated fatty acids around 30%. Date had dose-dependent parameters (curvilinear velocity [VCL] mainly) rabbit, although null or negative recorded doses >DPP40. Conversely, optimal conditions (i.e. semen), addition no traits (motility VCL). progressive increase DPP, despite providing additional amount tocols, caused higher tocol consumption oxidation. particular, DPP80 increased TBARS level both ages. better trend found DPP20, where figures 10.46 % 15.28 %, respectively, bucks, oxidation lower compared to conclusion, findings this study demonstrated supplementation extender enhanced chilled semen only if administered concentration than 40 mg/mL.
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ژورنال
عنوان ژورنال: World Rabbit Science
سال: 2023
ISSN: ['1989-8886', '1257-5011']
DOI: https://doi.org/10.4995/wrs.2023.18703